Introduction
This guide provides detailed instructions on how to set up and run the BD Rhapsody™ Sequence Analysis Pipeline on the Seven Bridges Genomics platform or on a local server installation.
This pipeline performs analysis of single-cell multiomic sequence read (FASTQ) data. The supported sequencing libraries are those generated by the BD Rhapsody™ assay kits, including: Whole Transcriptome mRNA, Targeted mRNA, AbSeq Antibody-Oligonucleotides, Single-Cell Multiplexing, TCR/BCR, and ATAC-Seq.
Included is a comprehensive reference to help you understand the pipeline's step-by-step process, the analysis algorithms, the output files, and the metrics. For any problems, please refer to the included Troubleshooting guide, or reach out to the support team.