Bioproduct Statistics


File: [sample_name]_Bioproduct_Stats.csv

The molecular identifier adjustment algorithms RSEC and DBEC are applied to each bioproduct. The molecular identifier metrics file lists the metrics from RSEC and DBEC on a per-bioproduct basis. For more information on RSEC and DBEC molecular identifier adjustment algorithms, see Molecules and error correction. For example:

MetricDefinition
BioproductBioproduct names listed in alphabetical order
Raw_ReadsNumber of reads before molecular identifier adjustment algorithms
Raw_MoleculesNumber of UMIs before molecular identifier adjustment algorithms
Raw_Seq_DepthNumber of raw reads ÷ the number of raw molecules
RSEC_Adjusted_MoleculesNumber of molecules detected after RSEC molecular identifier adjustment algorithm
RSEC_Adjusted_Reads_non-singletonNumber of RSEC-adjusted reads from molecules represented by more than one read
RSEC_Adjusted_Molecules_non-singletonNumber of RSEC-adjusted molecules represented by more than one read
RSEC_Adjusted_Seq_DepthNumber of raw reads ÷ the number of RSEC-adjusted molecules
DBEC_Adjusted_ReadsNumber of reads retained after DBEC molecular identifier adjustment algorithm
DBEC_Adjusted_MoleculesNumber of molecules retained after RSEC and DBEC
DBEC_Minimum_DepthThreshold of RSEC depth for a molecule to be considered a putative molecule by DBEC
DBEC_Adjusted_Seq_DepthNumber of DBEC-adjusted reads ÷ the number of molecules detected after RSEC and DBEC
DBEC_Depth_StatusBioproduct DBEC correction status:
  • Not detected: Bioproduct was not detected, because it has zero reads
  • Low depth: Minimum sequencing depth not achieved. DBEC not applied.
  • Pass: Minimum sequencing depth has been achieved