Bioproduct Statistics
File: [sample_name]_Bioproduct_Stats.csv
The molecular identifier adjustment algorithms RSEC and DBEC are applied to each bioproduct. The molecular identifier metrics file lists the metrics from RSEC and DBEC on a per-bioproduct basis. For more information on RSEC and DBEC molecular identifier adjustment algorithms, see Molecules and error correction. For example:
Metric | Definition |
---|---|
Bioproduct | Bioproduct names listed in alphabetical order |
Raw_Reads | Number of reads before molecular identifier adjustment algorithms |
Raw_Molecules | Number of UMIs before molecular identifier adjustment algorithms |
Raw_Seq_Depth | Number of raw reads ÷ the number of raw molecules |
RSEC_Adjusted_Molecules | Number of molecules detected after RSEC molecular identifier adjustment algorithm |
RSEC_Adjusted_Reads_non-singleton | Number of RSEC-adjusted reads from molecules represented by more than one read |
RSEC_Adjusted_Molecules_non-singleton | Number of RSEC-adjusted molecules represented by more than one read |
RSEC_Adjusted_Seq_Depth | Number of raw reads ÷ the number of RSEC-adjusted molecules |
DBEC_Adjusted_Reads | Number of reads retained after DBEC molecular identifier adjustment algorithm |
DBEC_Adjusted_Molecules | Number of molecules retained after RSEC and DBEC |
DBEC_Minimum_Depth | Threshold of RSEC depth for a molecule to be considered a putative molecule by DBEC |
DBEC_Adjusted_Seq_Depth | Number of DBEC-adjusted reads ÷ the number of molecules detected after RSEC and DBEC |
DBEC_Depth_Status | Bioproduct DBEC correction status: |